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recombinant Carboxypeptidase B E.coli,Carboxypeptidase B E.coli
Recombinant Carboxypeptidase B / Carboxypeptidase B / RCPB / Stable
DEscriptION
Recombinant Carboxypeptidase B
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the
C-terminal end of polypeptides. The molecular weight is 34,500 daltons, the pH optimum is 8.0, and pI is 6.0
Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal
chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B (EC 3.4.17.2) is expressed in E.Coli and
purified by high pressure liquid chromatography. There is no trace of other enzyme (such as
carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the
preparation.
ADVANTAGES
Animal origin free:YaxinBio recombinant carboxypeptidase B belongs to the AOF level 3. Level 3
recombinant carboxypeptidases B eliminate the risk of virus presence, or of any other potential
adventitious agents found in animal-derived carboxypeptitase B
Stable:A sterile recombinant carboxypeptidase B lyophilized powder eliminates the risk of contamination
and decreases the chances of activity loss in the process of transport and storage. It is also very convenient for transport and storage.
High purity:
1. Recombinant carboxypeptidase B provides increased specificity and eliminates contaminating activities
found in enzymes with lower purity level.
2. No other contaminating proteases such as chymotrypsin and carboxypeptidase A. Less than 10ppm of
recombinant trypsin.
3. One single main band at 35kD by SDS-PAGE.
