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Suppression subtractive hybridization (SSH) is one of the most powerful and popular PCR-based methods for isolating differentially expressed transcripts. Using a small quantity of either total RNA or poly(A)+ mRNA from each of two populations, the SSH procedure simultaneously subtracts and partially normalizes the abundance of target cDNA in the subtracted population. The clones are ligated into a vector, plated, picked, and sequenced. These genes are then available for analysis and further investigation by means of follow-on projects.More information, please visit the website: http://www.cd-genomics.com/SSH-cDNA-library-construction-service.html
SSH cDNA Library Construction Service
